General Cell Based Assays
Eurofins Discoverx PathHunter eXpress IL1R1/IL1RAP Dimerization Assay, 10pl Kit. (93-1032E3CP5L)
The PathHunter IL1R1/IL1RAP Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-1α) induced dimerization of IL1R1/IL1RAP receptors. This product uses EFC technology. The cell line included overexpresses IL1RAP target protein fused to PK alongside IL1R1 target protein fused to EA. Upon ligand-induced dimerization of IL1R1/IL1RAP, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Human BMP-7 ASY
The R-PLEX Human BMP-7 Assay Kit from Meso Scale Discovery is used to quantitatively measure BMP-7 levels in human serum plasma and cell culture supernatants It utilizes electrochemiluminescence (ECL) detection combined with patterned arrays The assay has high sensitivity and a broad dynamic range It includes a 96-well plate coated with capture antibody detection antibody read buffer wash buffer calibrator diluent and a vial of calibrator The kit has a measuring range of 1 95 - 2500 pg/mL and requires 25 L of sample
Eurofins Discoverx HitHunter cGMP Assay, 10,000 dp (384-well). (90-0039L)
The HitHunter cGMP Assay is a detection kit offering a robust, sensitive and easy-to-use assay to measure intracellular cGMP levels. This product uses EFC technology, and contains ED-tagged cGMP, anti-cGMP antibody, and pure EA. Upon application to a cell which contains endogenous, untagged cGMP, the anti-cGMP antibody will be sequestered by binding to this untagged cGMP, allowing for EFC between the tagged cGMP and soluble EA. This will produce a proportional level of chemiluminescent signal. This kit may be used with Eurofins DiscoverX cell lines as well as cell lines from other sources. This detection kit contains reagents for 25 plates (384 well). For detailed product information, visit www.discoverx.com/products-applications/biochemical-assays.
Eurofins Discoverx PathHunter eXpress C3AR1 U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0814E3CP0L)
The PathHunter C3AR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. C3a) based activation of C3AR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged C3AR1 and EA-tagged β-Arrestin-2. Activation of C3AR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mCCR6 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0694E2MCP2L)
The PathHunter mCCR6 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL20/MIP-3α) based activation of mCCR6 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mCCR6 and EA-tagged β-Arrestin-2. Activation of mCCR6 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress P2RY2 Activated GPCR Internalization Assay, 2pl Kit. (93-0719E3CP5M)
The PathHunter P2RY2 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of P2RY2 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged P2RY2. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting P2RY2-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery U-PLEX Mouse IL-16 ASY 25 PL
The Meso Scale Discovery U-PLEX Mouse IL-16 Assay is a multiplex immunoassay kit that enables simultaneous quantification of mouse interleukin 16 (IL-16) in serum plasma and cell culture supernatants This electrochemiluminescent assay utilizes MSD s proprietary SECTOR Imager and provides high sensitivity a wide dynamic range and requires low sample volume The kit includes a 96-well plate pre-coated with capture antibodies detection antibodies calibrators and controls Results are obtained in as little as 3 5 hours This assay allows for efficient reproducible analysis of IL-16 levels relevant to immunology and inflammation research
Meso Scale Discovery U-PLEX Mouse IL-6 ASY 25 PL
The Meso Scale Discovery U-PLEX Mouse IL-6 Assay Kit is a sensitive immunoassay for detecting mouse interleukin 6 (IL-6) in various samples It uses MSD s electrochemiluminescent technology offering high sensitivity a wide dynamic range and quick results The kit includes a 96-well plate with capture antibodies for mouse IL-6 detection antibodies with an electrochemiluminescent label and calibrators for a standard curve Sample incubation is 2 hours with no wash steps quantifying IL-6 from 0 2-10 000 pg/mL
Meso Scale Discovery RPLEX HUM Alphaamylase 1 ASY
The R-PLEX Human Alpha-Amylase 1 Assay Kit from Meso Scale Diagnostics is a multiplex immunoassay for measuring human alpha-amylase 1 in serum plasma saliva and cell culture supernatants It uses electrochemiluminescence (ECL) detection for high sensitivity broad dynamic range and rapid results The kit includes a 96-well plate with capture antibodies ECL-labeled detection antibodies calibrators controls diluent read buffer and wash buffer Sensitivity is 0 08 ng/mL with an upper quantification limit of 1500 ng/mL
Eurofins Discoverx PathHunter eXpress mPTGER4 DLD1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0982E13MCP25L)
The PathHunter mPTGER4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. prostaglandin E2) based activation of mPTGER4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mPTGER4 and EA-tagged β-Arrestin-2. Activation of mPTGER4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress P2RY2 Activated GPCR Internalization Assay, 10pl Kit. (93-0719E3CP5L)
The PathHunter P2RY2 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of P2RY2 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged P2RY2. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting P2RY2-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mCCR6 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0694E2MCP2M)
The PathHunter mCCR6 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL20/MIP-3α) based activation of mCCR6 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mCCR6 and EA-tagged β-Arrestin-2. Activation of mCCR6 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GLP1R Activated GPCR Internalization Assay, 2pl Kit. (93-0724E3CP0M)
The PathHunter GLP1R Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of GLP1R via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged GLP1R. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting GLP1R-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress TORC1 Nuclear Translocation Assay, 10pl Kit. (93-0833E3CP19L)
The PathHunter eXpress TORC1 Nuclear Translocation Assay provides an easy-to-use cell based assay to study drug candidates affecting the CRTC/CREB pathway. This kit assesses ligand (e.g. forskolin) based pathway activation via detection of resulting TORC1 (CRTC1) nuclear translocation. This product uses EFC technology. The included cell line overexpresses ProLabel-tagged TORC1 (CRTC1) and nuclear localized EA. Upon pathway activation, induced nuclear translocation of TORC1 leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter OSMRb/IL31R Bioassay Kit, 10-Plate. (93-1002Y3-00084)
The PathHunter OSMRb/IL31R Dimerization Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. IL-31) induced dimerization of OSMRb/IL31R receptors. This product uses EFC technology. The cell line included overexpresses OSMRb target protein fused to PK alongside IL31RA target protein fused to EA. Upon ligand-induced dimerization of OSMRb/IL31R, detectable EFC signal is produced. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.